Invited Talk World Congress DSA 2012

Quantitative Measurement of Cellular Events

Hualan Zhang
Institute of Computer Vision and applied Computer Sciences, IBaI, Leipzig, Germany, Project Consortium QuantPro

Abstract:

Cellular assays are highly recommended scientific tools in modern drug research and examination of pathological processes on cellular level.
Within the project QuantPro we developed based on innovative cell-based microscopy end-point assays a robust quantitative reference model for better understanding or quantitative measurements of dynamic or time dependent events in three key disease-relevant cellular pathways involving either internalisation (cell invasion or endocytosis of viruses or bacterial pathogens) or internal movements (mitochondria). Furthermore, we developed a suitable complex cellular functional experimental setup for drug screening in oncology which allows for microtiter plate-based screening modalities, multiple wavelength operation and high-resolution imaging. A highly innovative technology component for the application of a newly developed imaging system “Selective Plane Illumination Microscope (SPIM)” to extend the dynamic measurements made by two-dimensional microscopy and confocal scanning sections to 3D microscopy by confocal theta microscopy. Novel bioinformatic and software solutions were develped to evaluate both cellular models in a dynamic 3D mode. The talk summarizes the results of the project QuantPro. The project consortium has been comprised of five working groups MPI-CBG Dresden, Schering AG, EMBL group Stelzer, Institute of Computer Vision and applied Computer Sciences, IBaI, and EMBL group Bork.

 

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